Targeting of polymer drug delivery systems with recombinant proteins bound via tris(nitrilotriacetic acid) and coiled coil

Michal Pechar
Lecture of the lecture cycle
24.10.2024 10:30, Lecture room A

Antibody-mediated targeting is an efficient strategy to enhance the specificity and selectivity of polymer nanomedicines towards the target site, typically a tumor. However, direct covalent coupling of an antibody with a polymer usually results in a partial damage of the antibody binding site accompanied with a compromised biological activity. We developed an original solution based on well-defined non-covalent interactions between tris-nitrilotriacetic acid (trisNTA) and hexahistidine (His-tag) groups, purposefully introduced to the structure of each macromolecule. Specifically, trisNTA groups were attached to a copolymer based on N-(2-hydroxypropyl)methacrylamide (HPMA), and to a thermo-responsive di-block copolymers based on N-isopropylmethacrylamide (NIPMAM) and HPMA; His-tag was incorporated to the structure of a recombinant single chain fragment of an anti-GD2 monoclonal antibody (scFv-GD2). Flow cytometry and fluorescence microscopy clearly demonstrated anti-gen-specific binding of the prepared complexes to GD2 positive cell lines.

The lecture is presented in English