| Description |
The goal of the dissertation is the development of a new concept of a potentiometric sensor based on polymer detection layers, usable for the detection of markers of bacterial and sterile inflammation, the presence of endotoxins, or polyvalent toxic metal ions. The student will develop knowledge of polymer synthesis, master the technology of applying polymer sensing layers and their characterization by instrumental methods, such as potentiometry and cyclic voltammetry, spectrofluorometry (steady-state, time-resolved), confocal microscopy, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and others. Part of the work will be testing the functionality of the prepared detection layers first on synthetic analytes. The obtained results will be used to optimize the polymer electrodes, which will subsequently be tested on real biological or environmental samples. The student will use the new knowledge to design a multisensor electrode concept. The topic of the thesis is highly interdisciplinary, includes a number of methodologies and can be further adjusted according to the individual interests of the student. The application potential of the achieved results will be verified within the framework of cooperation with the workplaces of university hospitals. |
